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Metabolic substrates are not mobilized from the osmoregulatory organs (gills and kidney) of the estuarine pufferfishes Sphoeroides greeleyi and S. testudineus upon short-term salinity reduction Neotropical Ichthyology
Prodocimo,Viviane; Souza,Carine F.; Pessini,Cristina; Fernandes,Luiz Claudio; Freire,Carolina A..
The marine-estuarine species of pufferfishes Sphoeroides testudineus and S. greeleyi are very efficient osmoregulators. However, they differ with respect to their tolerance of salinity reduction. During low tide S. testudineus remains in diluted estuarine waters, whereas S. greeleyi returns to seawater (SW). The hypothesis tested here was that the short-term mobilization of metabolic substrates stored in their main osmoregulatory organs would correlate with this differential tolerance. Fishes exposed to 5‰ (for 6 h) were compared to those kept in 35‰. Branchial and renal contents of triglycerides, protein and glycogen were evaluated, and total ATPase activity accounted for the tissues' metabolism. Plasma osmolality, chloride and glucose, hematocrit, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish gill; Fish kidney; Glycogen; Metabolism; Osmoregulation; Tetraodontidae.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252008000400009
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Molecular characterisation of prolactin and analysis of extrapituitary expression in the European sea bass Dicentrarchus labrax under various salinity conditions ArchiMer
Boutet, Isabelle; Lorin Nebel, C; De Lorgeril, Julien; Guinand, B.
dAlthough prolactin has been demonstrated to be the main hormone involved in adaptation to dilute media in several freshwater teleosts, few studies have been conducted in marine teleosts. In the Mediterranean, the sea bass Dicentrarchus labrax inhabits environments ranging from the open sea to coastal lagoons, where salinity varies greatly. We characterised the prolactin (prl) gene and analysed its expression in two organs (gill and intestine) in D. labrax acclimated to either freshwater or seawater. A 2819 bp long sequence encompassing the prl gene and a part (282 bp) of the promoter were identified, and these comprised 5 coding exons separated by 4 introns. Prolactin was similarly expressed in fresh- and seawater adapted fish, although expression in...
Tipo: Text Palavras-chave: Prolactin; Phylogenctic analysis; MRNA expression; Osmoregulation; Intestine; Gill; Dicentrarchuslabrax.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2537.pdf
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Ontogeny of osmoregulation in the Pacific blue shrimp, Litopenaeus stylirostris (decapoda, penaeidae): Deciphering the role of the Na+/K+-ATPase ArchiMer
Pham, Dominique; Charmantier, Guy; Boulo, Viviane; Wabete, Nelly; Ansquer, Dominique; Dauga, Clement; Grousset, Evelyse; Labreuche, Yannick; Charmantier-daures, Mireille.
The role of the main ion transporting enzyme Na +/K + − ATPase in osmoregulation processes was investigated in Litopenaeus stylirostris. The development and localization of the osmoregulation sites were studied during ontogenesis by immunodetection of Na+ K+-ATPase using monoclonal antibodies and transmission electron microscopy (TEM). Osmoregulation sites were identified as the pleurae and branchiostegites in the zoeae and mysis stages. In the subsequent post-metamorphic stages the osmoregulatory function was mainly located in the epipodites and branchiostegites and osmotic regulation was later detected in the gills. The presence of ionocytes and microvilli in these tissues confirmed their role in ionic processes. The complete open reading frame of the...
Tipo: Text Palavras-chave: Penaeid; Litopenaeus stylirostris; Ontogeny; Osmoregulation; Na+/K+-ATPase; RNA interference.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00311/42176/41477.pdf
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Osmoregulation and salinity-induced oxidative stress: is oxidative adaptation determined by gill function? ArchiMer
Rivera-ingraham, Georgina A.; Barri, Kiam; Boel, Melanie; Farcy, Emilie; Charles, Anne-laure; Geny, Bernard; Lignot, Jehan-herve.
Osmoregulating decapods such as the Mediterranean green crab Carcinus aestuarii possess two groups of spatially segregated gills: anterior gills serve mainly respiratory purposes, while posterior gills contain osmoregulatory structures. The co-existence of similar tissues serving different functions allows the study of differential adaptation, in terms of free radical metabolism, upon salinity change. Crabs were immersed for 2 weeks in seawater (SW, 37 ppt), diluted SW (dSW, 10 ppt) and concentrated SW (cSW, 45 ppt). Exposure to dSW was the most challenging condition, elevating respiration rates of whole animals and free radical formation in hemolymph (assessed fluorometrically using C-H(2)DFFDA). Further analyses considered anterior and posterior gills...
Tipo: Text Palavras-chave: Antioxidant defense; Carcinus aestuarii; ROS production; Osmoregulation.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00626/73816/74410.pdf
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Osmoregulatory responses to dietary protein and water intake in the granivorous Zonotrichia capensis (Passerine, Emberizidae) RChHN
ALDEA,PATRICIA; SABAT,PABLO.
We studied the osmoregulatory responses of the granivore Zonotrichia capensis, acclimated to high- and low-protein diets as well as two levels of water intake. We tested whether Z. capensis has the ability to modify the proportion of nitrogenous waste in the excreta when protein intake and water intake varies. After 21 days of acclimation, plasma osmolality was not significantly affected by dietary treatment; however, excreta osmolality was higher in the high-protein group compared to the low-protein group. Nitrogenous wastes in Z. capensis are excreted mainly in the form of uric acid. The proportion of kidney devoted to medullary tissue was 40 % higher in dehydrated birds than in hydrated birds. Excreta osmolality was higher in dehydrated birds, and in...
Tipo: Journal article Palavras-chave: Granivory; Kidney; Nitrogenous waste; Osmoregulation; Passerines.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2007000400006
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Oxygen consumption remains stable while ammonia excretion is reduced upon short time exposure to high salinity in Macrobrachium acanthurus (Caridae: Palaemonidae), a recent freshwater colonizer Rev. Bras. Zool.
Freire,Carolina A.; Rios,Leonardo de P.; Giareta,Eloísa P.; Castellano,Giovanna C..
ABSTRACT Palaemonid shrimps occur in the tropical and temperate regions of South America and the Indo-Pacific, in brackish/freshwater habitats, and marine coastal areas. They form a clade that recently (i.e., ~30 mya) invaded freshwater, and one included genus, Macrobrachium Bate, 1868, is especially successful in limnic habitats. Adult Macrobrachium acanthurus (Wiegmann, 1836) dwell in coastal freshwaters, have diadromous habit, and need brackish water to develop. Thus, they are widely recognized as euryhaline. Here we test how this species responds to a short-term exposure to increased salinity. We hypothesized that abrupt exposure to high salinity would result in reduced gill ventilation/perfusion and decreased oxygen consumption. Shrimps were subjected...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ammonia; Lactate; Osmoregulation; Palaemonidae.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702017000100323
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Physiological and biochemical changes of wild and cultivated juvenile pink shrimp Farfantepenaeus duorarum (crustacea: penaeidae) during molt cycle ArchiMer
Luis Bonilla-gomez, Jose; Chiappa-carrara, Xavier; Galindo, Carmen; Jeronimo, Gilberto; Cuzon, Gerard; Gaxiola, Gabriela.
Changes in metabolite levels in hemolymph and hepatopancreas were used as indicators of physiological status of juvenile wild and cultivated Farfantepenaeas. duorarum (Burkenroad, 1939), during hyper-osmoregulatory conditions (salinity 22 ppt, 726 mOsm/kg). We analyzed the relationship between biochemical changes of wild and cultivated shrimp at different molt stages by measurement of osmotic capacity, hemocyanin, acylglycerides, cholesterol, glucose, glycogen and total protein. Biochemical analyses of hemolymph (blood) and hepatopancreas showed a decreasing trend in stages closer to molt and an increasing trend before intermolt. Osmotic capacity and blood proteins indicated a significant effect of the molt stage. Stages A. D1' and D''' showed the greatest...
Tipo: Text Palavras-chave: Biochemical analysis; F. duorarum; Molt stages; Osmoregulation.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00088/19936/17773.pdf
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Physiological basis of interactive responses to temperature and salinity in coastal marine invertebrate: Implications for responses to warming ArchiMer
Torres, Gabriela; Charmantier, Guy; Wilcockson, David; Harzsch, Steffen; Giménez, Luis.
Developing physiological mechanistic models to predict species’ responses to climate‐driven environmental variables remains a key endeavor in ecology. Such approaches are challenging, because they require linking physiological processes with fitness and contraction or expansion in species’ distributions. We explore those links for coastal marine species, occurring in regions of freshwater influence (ROFIs) and exposed to changes in temperature and salinity. First, we evaluated the effect of temperature on hemolymph osmolality and on the expression of genes relevant for osmoregulation in larvae of the shore crab Carcinus maenas. We then discuss and develop a hypothetical model linking osmoregulation, fitness, and species expansion/contraction toward or away...
Tipo: Text Palavras-chave: Carcinus maenas; Climate change; Coastal zone; Larva; MRNA expression; Multiple stressors; Osmoregulation; Salinity; Temperature.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00692/80408/83516.pdf
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Physiological effects of gasoline on the freshwater fish Prochilodus lineatus(Characiformes: Prochilodontidae) Neotropical Ichthyology
Simonato,Juliana D.; Fernandes,Marisa N.; Martinez,Cláudia B. R..
The purpose of this work was to evaluate the effects of the water-soluble fraction of gasoline (WSFG) on the Neotropical freshwater fish Prochilodus lineatus. The WSFG was prepared by mixing gasoline in water (1:4) and animals were exposed for 6, 24 and 96h to 5% diluted WSFG or only to water. After exposure, blood was collected from the caudal vein and the gills were removed. The following parameters were analyzed: hematological (hemoglobin, hematocrit, number of red blood cells), osmo-ionic (plasma Na+, Cl- and K+ and plasma osmolarity), metabolic (total plasma proteins and glucose), endocrine (cortisol), density and distribution of chloride cells [CC] in the gills (immunohistochemistry), and branchial Na+/K+-ATPase (NKA) activity. Hemolysis was found to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chloride cells; Hematology; Hydrocarbons; Na+K+-ATPase; Osmoregulation; Stress response.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300683
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Physiological responses of two ecologically important Kenyan mangrove crabs exposed to altered salinity regimes OceanDocs
De Wachter, B.; Gillikin, D.P.; Tack, J..
The potential long-term effects of altered salinity regimes on the bioenergetics of two ecologically important Kenyan mangrove crabs, Neosarmatium meinerti de Man, 1887 and Neosarmatium smithi H. Milne Edwards, 1853 were investigated in light of recent findings suggesting that groundwater redirection may alter salinity regimes in Kenyan mangroves. Although changes in groundwater may cause only small increases in salinities, these changes would be chronic and may impact crab populations already living above their optimal salinity. To assess potential impacts, fundamental physiological processes and hemolymph components were measured on animals acclimated to 16‰, 32‰, 48‰ and 65‰ for 4 weeks in a field laboratory. For comparative purposes, crabs were also...
Tipo: Journal Contribution Palavras-chave: Ecophysiology; Salinity Tolerance; Energy Budget; Osmoregulation; Sesarmidae; Crab fisheries.
Ano: 2004 URL: http://hdl.handle.net/1834/693
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Plasma ion levels of freshwater and marine/estuarine teleosts from Southern Brazil Neotropical Ichthyology
Becker,Alexssandro Geferson; Gonçalves,Jamile Fabbrin; Toledo,Jaderson dos Anjos; Burns,Marcelo D. M.; Garcia,Luciano de Oliveira; Vieira,João Paes; Baldisserotto,Bernardo.
The purpose of this study was to investigate Na+, Cl-, K+, Ca2+, and Mg2+ levels in the plasma of freshwater and marine/estuarine teleosts collected at different salinities (0 to 34) from the estuarine and freshwater portions of the São Gonçalo channel in Southern Brazil. Any relationship between plasma ion levels and salinity and the capacity of ionic regulation of teleosts found at three or more different salinities (Genidens barbus and Micropogonias furnieri) was also investigated. Results showed no relationship between plasma ion levels and salinity when considering all species together, but the two species collected from three or more different salinities showed a significant positive relationship between plasma ion levels and salinity, indicating...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ariidae; Estuaries; Ionoregulation; Osmoregulation; Salinity; Sciaenidae.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000400021
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Proline and trehalose in maize seeds germinating under low osmotic potentials AGRIAMBI
Queiroz,Rafaela J. B.; Cazetta,Jairo O..
ABSTRACT Although it is relatively well known that adult plants tend to accumulate proline and trehalose in their tissues as a physiological mechanism in response to drought, there is scarce information about the development of this physiological response in seeds. Thus, the objective of this research was to verify if maize seeds are able to develop mechanism of osmoprotection, when are germinating under low osmotic potential, and the possibility to use the levels of trehalose and proline in a defined seed part, aiming to differentiate genotypes regarding drought tolerance. The experiment was performed as a factorial arrangement of 2 x 5 (2 hybrids x 5 osmotic potential) within a completely randomized design, with four replicates. It was found that the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays; Germination; Water restriction; Osmoregulation; Compatible solutes.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662016000100022
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Response of the blue shrimp Litopenaeus stylirostris to temperature decrease and hypoxia in relation to molt stage ArchiMer
Mugnier, Chantal; Soyez, Claude.
The effect of different levels of dissolved oxygen (DO) and a decreased temperature combined with severe hypoxia were studied in the blue shrimp Litopenaeus stylirostris in relation to molt stage. In the first experiment, shrimps were submitted to DO concentrations of 5 to 1 mg l(-1). Osmoregulatory capacity (OC) was measured in shrimps at molt stage C after 6, 24, or 48 h of exposure. No mortality was recorded but a significant negative effect of DO concentration on hypo-OC was observed for DO concentrations below 3 mg l(-1). Osmoregulation improved after 48 h exposure, compared to 6 and 24 h exposure. In a second experiment, temperature was decreased from 28 to 22 degreesC in 24 h, before a severe hypoxia of 0.5+/-0.1 mg O-2 l(-1) was applied....
Tipo: Text Palavras-chave: Temperature; Stress; Penaeid shrimp; Osmoregulation; Molt stage; Litopenaeus stylirostris; Hypoxia.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-372.pdf
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Salinity stress from the perspective of the energy-redox axis: Lessons from a marine intertidal flatworm ArchiMer
Rivera-ingraham, Georgina A.; Nommick, Aude; Blondeau-bidet, Eva; Ladurner, Peter; Lignot, Jehan-herve.
In the context of global change, there is an urgent need for researchers in conservation physiology to understand the physiological mechanisms leading to the acquisition of stress acclimation phenotypes. Intertidal organisms continuously cope with drastic changes in their environmental conditions, making them outstanding models for the study of physiological acclimation. As the implementation of such processes usually comes at a high bioenergetic cost, a mitochondrial/oxidative stress approach emerges as the most relevant approach when seeking to analyze whole-animal responses. Here we use the intertidal flatworm Macrostomum lignano to analyze the bioenergetics of salinity acclimation and its consequences in terms of reactive oxygen/nitrogen species...
Tipo: Text Palavras-chave: Energetic balance; Flatworms; Live-imaging; Mitochondrial membrane potential; Osmoregulation; RNS; ROS.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00625/73743/74411.pdf
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Salinity tolerance, ontogeny of osmoregulation and zootechnical improvement in the larval rearing of the Caledonian Blue Shrimp, Litopenaeus stylirostris (Decapoda, Penaeidae) ArchiMer
Pham, Dominique; Charmantier, Guy; Wabete, Nelly; Boulo, Viviane; Broutoi, Francis; Mailliez, Jean-rene; Peignon, Jean-marie; Charmantier-daures, Mireille.
The ontogeny of osmoregulationwas investigated in Litopenaeus stylirostris by studying salinity tolerance and osmoregulatory capacity. Shrimp at different larval and postlarval stages were exposed to various salinities and survival was monitored for 24 h. Survival rates exceeded 80% at salinity over 25 ppt (750 mOsm.kg(-1)) at all the stages. At salinities below to 25 ppt, salinity tolerance was higher in nauplii and zoeae than in mysis larvae. Postlarval stages were able to withstand lower salinities, e.g. 6.0 ppt (176 mOsm.kg(-1)) at PL9 stage, but they were more sensitive than larvae to salinities over 35 ppt (1035 mOsm.kg(-1)). Zoea and mysis larvae slightly hyper-regulated at all tested salinities. After metamorphosis, postlarvae progressively...
Tipo: Text Palavras-chave: Penaeid; Litopenaeus stylirostris; Ontogeny; Osmoregulation; Growth.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00107/21843/20058.pdf
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Salt concentrations in culture media for the development of Dipteryx alata in vitro PAB
Araruna,Elequisandra da Costa; Ribeiro-Oliveira,João Paulo; Pereira,Vanderley José; Asmar,Simone Abreu; Melo,Berildo de.
Abstract: The objective of this work was to evaluate how salt concentrations in media used for in vitro cultures affect the early development of “barueiro” (Dipteryx alata) seedlings obtained from the shoot apex. Two different in vitro culture media - Murashige & Skoog (MS) and wood plant medium (WPM) - in different salt concentrations (25, 50, 75, and 100%) were used. At 120 days, the D. alata seedlings in the MS medium, in its original concentration (100%), developed better than in other concentrations or in the WPM. Therefore, the MS medium was considered the most suitable for ensuring in vitro establishment because it provided greater root length (27.65 cm) and number of leaves per plant (26 leaves) than the other medium.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerrado biome; Culture media; Osmoregulation; Plant growth; Salt concentrations; Tissue culture.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001201295
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Seasonal changes in the use of marine food resources by Cinclodes nigrofumosus (furnariidae, aves): carbon isotopes and osmoregulatory physiology RChHN
SABAT,PABLO; MARTÍNEZ DEL RÍO,CARLOS.
Passerines lack functional salt glands and to a large extent avoid feeding on marine invertebrates. An exception is Cinclodes nigrofumosus. Previous studies reported that the contents of its gastrointestinal tract had a lower osmolality than seawater suggesting that birds were supplementing their marine invertebrate diet with terrestrial invertebrates and fresh water at certain periods of the year. We report seasonal changes in the diet of C. nigrofumosus at two contrasting coastal localities in Central Chile and document the potential correlation between weather and feeding habits with osmoregulatory features in this species. We expected that C. nigrofumosus would increase its use of osmotically challenging marine dietary sources when fresh water is...
Tipo: Journal article Palavras-chave: Cinclodes; Isotopes; Renal function; Osmoregulation; Seasonal changes.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2005000200009
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Terrestrial birds living on marine environments: does dietary composition of Cinclodes nigrofumosus (Passeriformes: Furnariidae) predict their osmotic load? RChHN
SABAT,PABLO; FARIÑA,JOSÉ M.; SOTO-GAMBOA,MAURICIO.
Feeding on saline marine foods may be especially challenging for passerine birds that lack functional salt glands and have a limited ability to concentrate urine. To reduce the salt load imposed by consumption of marine food these birds may select food with low salt contents and/or increase their intake of freshwater. The genus Cinclodes is particular among passerines because it includes species that inhabit both inland and maritime shores. We analyzed the diet of Cinclodes nigrofumosus and explored the possible relationships between dietary composition and salt load at mesic and arid coastal sites of Chile. From a biogeographical perspective, we hypothesized that freshwater availability is critical to the relationship between diet composition and osmotic...
Tipo: Journal article Palavras-chave: Cinclodes; Dietary habits; Osmoregulation; Osmotic load.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2003000200017
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The ClC-3 chloride channel and osmoregulation in the European Sea Bass, Dicentrarchus labrax ArchiMer
Bossus, Maryline; Charmantier, Guy; Blondeau-bidet, Eva; Valletta, Bianca; Boulo, Viviane; Lorin-nebel, Catherine.
Dicentrarchus labrax migrates between sea (SW), brackish and fresh water (FW) where chloride concentrations and requirements for chloride handling change: in FW, fish absorb chloride and restrict renal losses; in SW, they excrete chloride. In this study, the expression and localization of ClC-3 and Na+/K+-ATPase (NKA) were studied in fish adapted to SW, or exposed to FW from 10 min to 30 days. In gills, NKA-alpha 1 subunit expression transiently increased from 10 min and reached a stabilized intermediate expression level after 24 h in FW. ClC-3 co-localized with NKA in the basolateral membrane of mitochondria-rich cells (MRCs) at all conditions. The intensity of MRC ClC-3 immunostaining was significantly higher (by 50 %) 1 h after the transfer to FW,...
Tipo: Text Palavras-chave: ClC-3 chloride channel; Na+/K+-ATPase; Dicentrarchus labrax; Osmoregulation; Osmosensing.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00149/26044/25185.pdf
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The effects of low salinity on lipid composition in the gills of grey mullet "Liza ramada" OceanDocs
Rabeh, I.; Telahigue, K.; Chetoui, I.; Masmoudi, W.; El Cafsi, M..
Les alevins de Liza ramada adaptés à l’eau de mer (EM, 35ppt) ont subi au laboratoire un stress halin par transfert direct de l’eau de mer à l’eau douce (ED, 0,5 ppt) pendant 24 heures. Les aspects hématologiques ainsi que la composition lipidique du tissu branchial des alevins ont été suivis. Le transfert à la basse salinité s’est accompagné d’une diminution significative de l’osmolalité du plasma, du taux d’hématocrite et du nombre des globules rouges. Le suivi de l’évolution des lipides des branchies des alevins en fonction de la salinité du milieu, montre une augmentation des acides gras monoinsaturés, particulièrement l’acide oleique C18 :1(n-9) à la basse salinité. Une réduction des pourcentages des acides gras polyinsaturés de la famille...
Tipo: Journal Contribution Palavras-chave: Osmoregulation; Mullet fisheries; Osmotic adaptations.
Ano: 2010 URL: http://hdl.handle.net/1834/4701
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